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Publications by the Minor laboratory have been cited over 31,000 times according to Web of Knowledge (over 37,500 times according to Google Scholar).

The paper describing the HKL suite is #23 on the list of the 100 most cited scientific papers of all-time
(#7 among papers published in the last 20 years)

Wladek Minor, Ph.D.
 Professor of Molecular Physiology and Biological Physics


Phone - Office:  (434) 243-6865
Phone - Lab:  (434) 924-2948
Fax:  (434) 243-2981
Directions to the laboratory (in PDF format)

FedEx Address:

Department of Molecular Physiology
and Biological Physics
University of Virginia
1340 Jefferson Park Avenue,
Jordan Hall
Room 4223
Charlottesville, VA 22908

US Mail Address:

Department of Molecular Physiology
and Biological Physics
University of Virginia
PO Box 800736
Charlottesville, VA 22908-0736

Research Interests:

Our laboratory studies macromolecular structure with an aim toward in-depth understanding of structure-function relationships. X-ray diffraction analysis is our primary research tool, but we also employ other physical and biochemical methods of analysis. The program emphasizes two broad themes; crystallographic studies on molecules of immediate interest, and methodology development. Most of the macromolecules that we have under study relate to one or more of a few broad biological areas: cellular signal transduction and metalloproteins. The same systems have been chosen as subjects for methodology development. The methodology development includes the development of various crystallographic tools that create the HKL Package.

Another research area is high-throughput crystallography and structural genomics. Our lab is involved in a number of large, biomedically oriented projects that will revolutionize biomedical research in this decade. We are a member of the Midwest Center for Structural Genomics and the New York Structural Genomics Research Consortium (both centers of the NIH Protein Structure Initiative), and the Center for Structural Genomics of Infectious Disease (a project of the NIAID). We are also a part of the Enzyme Function Initiative (an NIH Glue Grant). We develop widely used methodology that is used in thousands of structural biology laboratories around the world. We collaborate with many synchrotron beamlines, in particular, with the Structural Biology Center at the Advanced Photon Source, and with many individual laboraries.

These collaborations have been exceptionally fruitful, resulting in joint publications with researchers from 79 different institutions from around the world. See an interactive map of the geographical distribution of these institutions.

The lab is well equipped to facilitate large scale protein purification, crystallization, biophysical characterization and detection of protein/protein or protein/small molecule interactions.

Wladek Minor's Webinars

   SBGrid -- HKL-2000 January 2012

Web Services

  Check My Metal -- A web server for the validation of metal binding sites in macromolecules.

Lab Members

David Cooper, Instructor,
Marcin Cymborowski, Research Assistant,
Jose Font, Visiting Faculty,
Olga Gasiorowska, Visiting Graduate Student,
Marek Grabowski, Research Scientist,
Katarzyna Handing, Visiting Graduate Student,
Jing Hou, Research Scientist,
Marcin Kowiel, Visiting Graduate Student,
Karol Langner, Research Associate,
Cody LaRowe, Undergraduate Student,
Karolina Majorek, Visiting Graduate Student,
Darryl Mason, Undergraduate Student,
Tomasz Osinski, Visiting Graduate Student,
Ivan Shabalin, Research Associate,
Igor Shumilin, Instructor,
Piotr Sroka, Visiting Graduate Student,
Heping Zheng, Data Scientist,
Matthew Zimmerman, Instructor,

Former Lab Members

   Maks Chruszcz, Associate Professor, University of South Carolina, Columbia, SC
   Krzystof Lewinski, Professor, Jagellonian University, Krakow, Poland
   Hai Bin Luo , Professor, Research Center for Drug Discovery, Sun Yat-Sen University, Guangzhou, China
   Diana Tomchick, Professor, UT Southwestern Medical Center, Dallas, TX
   James Spencer, Senior Lecturer in Microbial Pathogenesis, University of Bristol, Bristol, UK
   Ekaterina Filippova, Research Associate, Northwestern University, Chicago, IL
   Katarzyna Koclega, Wroclaw Research Centre EIT+, Wroclaw, Poland
   Janusz Petkowski, Research Associate, ETH Zurich, Switzerland
   Aleksandra Knapik, Graduate Student, Department of Cytobiochemistry, University of Lodz
   Alexandra Kos (Zalcman), Nexus Telecom, Zurich, Switzerland
   Robert Nicholls, Graduate Student, University of York, York, UK
   Haijun Qiu, Database Administrator, The Children's Hospital of Philadelphia, Philadelphia, PA
   John Raynor, Structural Genomics Consortium, Oxford University, Oxford, UK
   Pawel Sledz, Gates Scholar, University of Cambridge, Cambridge, UK
   Shuren Wang, Technical Data Manager, OpenQ Inc., Charlottesville, VA
   Arjun Dayal, Co-founder, Monte Carlo Software LLC


  bullet A list of Minor Lab alumni


ISGO Meeting, Beijing, October 2006
Lab Holiday Party, UVA, December 2003

Selected Publications

  1. Zimmerman MD, Proudfoot M, Yakunin A, Minor W  (2008)
    Structural Insight into the Mechanism of Substrate Specificity and Catalytic Activity of an HD-Domain Phosphohydrolase: The 5'-Deoxyribonucleotidase YfbR from Escherichia coli
    Journal of Molecular Biology 378: 215-26.  Times cited: 33.  [Pub Med ID: 18353368] [Pub Med Central ID: 2504004]

  2. Kirillova O, Chruszcz M, Shumilin IA, Skarina T, Gorodichtchenskaia E, Cymborowski M, Savchenko A, Edwards A, Minor W  (2007)
    An Extremely SAD Case: Structure of a Putative Redox-Enzyme Maturation Protein from Archaeoglobus Fulgidus at 3.4 Angstrom Resolution
    Acta Crystallographica Section D: Biological Crystallography 63: 348-54.  Times cited: 21.  [Pub Med ID: 17327672]

  3. Minor W, Cymborowski M, Otwinowski Z, Chruszcz M  (2006)
    HKL-3000: The Integration of Data Reduction and Structure Solution--From Diffraction Images to an Initial Model in Minutes
    Acta Crystallographica Section D: Biological Crystallography 62: 859-66.  Times cited: 444.  [Pub Med ID: 16855301]

  4. Otwinowski Z, Borek D, Majewski W, Minor W  (2003)
    Multiparametric scaling of diffraction intensities
    Acta Crystallographica. Section A: Foundations of Crystallography 59: 228-34.  Times cited: 481.  [Pub Med ID: 12714773]

  5. Otwinowski Z, Minor W  (1997)
    Processing of X-ray diffraction data collected in oscillation mode
    Methods in Enzymology 276: 307-326.  Times cited: 29298.  

  6. Minor W, Steczko J, Stec B, Otwinowski Z, Bolin JT, Walter R, Axelrod B  (1996)
    Crystal structure of soybean lipoxygenase L-l at 1.4 angstrom resolution
    Biochemistry 35: 10687-701.  Times cited: 311.  [Pub Med ID: 8718858]

  7. Minor W, Steczko J, Bolin JT, Otwinowski Z, Axelrod B  (1993)
    Crystallographic Determination Of The Active-Site Iron And Its Ligands In Soybean Lipoxygenase L-1
    Biochemistry 32: 6320-3.  Times cited: 137.  [Pub Med ID: 8518276]

More publications with external links are available.

Last modified: Tue March 24, 2015 02:22:09 PM
Source: Web of Science©